TumorTACS™ In Situ Apoptosis Detection Kit

TUNEL assay cell type - PANC-1 human pancriatic cancer

Experiment
TUNEL assay cell type - PANC-1 human pancriatic cancer
Product
TumorTACS™ In Situ Apoptosis Detection Kit from Bio-Techne
Manufacturer
Bio-Techne

Protocol tips

Upstream tips
Warm slides to 57C for 5 minutes and treate with xylene and ethanol to deparaffinize
Protocol tips
Treat with Cytonin or Proteinase K and incubate at room temperature

Add Labeling Reaction Mix and incubate at 37 ˚C for 1 hour in a humidity chamber
Downstream tips
If no staining in TACS Nuclease, increase incubation time with Proteinase K (up to maximum 60 minutes) or Cytonin (up to overnight at 4 oC)

Publication protocol

TUNEL staining was performed by using Tumor TACS™ In Situ Apoptosis Detection Kit (Trevigen), the specimens were deparaffinised and labeled following the procedure provided by the manufacturer. Finally, DAB staining were visualized under microscopy [43]. For TUNEL assay, ten fields were randomly selected from each slide for measurement, the images were analyzed by MetaMorph software and presented as a percentage of the total number of cells [44].

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

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Manufacturer protocol

Download the product protocol from Bio-Techne for TumorTACS™ In Situ Apoptosis Detection Kit below.

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